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Mals produced antibodies which recognized gHt and gL on a Western blot of a denaturing gel (Fig. 5A). On a Western blot of a nondenaturing (native) gel (6), these antibodies also recognized higherTABLE 1. HSV neutralizing activity of sera from rabbits immunized with gHt-gLVirus neutralization titer (50 ) Adjuvant Rabbit Entry assay,a HSV-1(hrR3) Plaque assayb HSV-1(KOS) HSV-2(333)Freund's R136 R13

Once you reach the Route of the Hiawatha trailhead, you have two options. You can drive to the parking lot which begins at the big tunnel (which I'll talk about later) at the East Portal trailhead, or you can ride bypass this and drive to Roland trail head to avoid the dark tunnel. We chose to ride the whole trail, so we started at the East Portal.

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