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A reverse transcription using ,2 ug of poly(A)+ RNA template and oligo-(dT)12?8 primer using First Strand cDNA Synthesis kit (Novagen). The resulting cDNA was used as a template for PCR amplification using PfuUltra HighFidelity DNA Polymerase (Stratagene) and degenerated primers specific for FvH and FvL gene fragments (Mouse Ig-Primer Set, Novagen). PCR timing has been set according to the manufac
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