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Precipitated by adding protein A-Sepharose. After subjecting the precipitate to SDS-PAGE, 32 and DnaK were detected by CBB staining, and DnaJ was detected by immunoblotting with anti-DnaJ serum. Pull-down assays for the interaction between 32 and DnaKHis or DnaJ-His were performed using Ni2 -NTA-agarose. DnaK-His was preincubated in Buffer D (20 mM Tris-HCl (pH 7.5), 100 mM KCl, 10 glycerol, 5 mM
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Derived from the requirements of individuals learners, you can alter the degree of spontaneous and structured observe.
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The Mexican Authorities is forcing their most undesirable individuals into the United States.
Challenging a sitting president and shedding shouldn't be an amazing mark to have on one's political career.
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